RTMNU Syllabus

RTMNU Revised BSc Microbiology Sem 6 Syllabus 2021


RTMNU Revised BSc Microbiology Sem 6 Syllabus 2021

RTMNU  BSc Microbiology Revised Sem 6 Syllabus 2020-21 | Nagpur University B.Sc Microbiology Syllabus Part 3 | RTMNU Third Year B.Sc. Microbiology Syllabus 

Nagpur University New Syllabus 2021 is available for Downloading. The latest RTMNU Revised Bsc Microbiology  Sem 6 Syllabus 2021 is published by Nagpur University. The students looking for this syllabus can Download the PDF Syllabus from given respective Links. We keep adding More details about this Syllabus on this page. We given below the Details updated new syllabus. Students of RTMNU are requested to go though the detail syllabus. You can also download the PDF of syllabus from given link. For More updates keep visiting us.

RTM Nagpur University Revised B.Sc Microbiology Sem 6 Syllabus 2021 -RTM Nagpur University B.Sc Microbiology Sixth Semester New Revised Syllabus is given below for Downloading. The students can Download the respective Syllabus from following given details. Just go through the given links & read the given syllabus carefully. Nagpur University Third Year New Semester Online Detail syllabus given below.

Syllabus B.Sc. Part- III Microbiology

PAPER I IMMUNOLOGY

Unit-I Immunity and Non specific defenses

1. Immunity: Definition and general concept
2. Haematopoiesis and Cells of immune system
a) Diagram of Haematopoiesis
b) General characteristics of
i. B and T cells,
ii. Monocytes and macrophages,
iii. Neutrophils, Eosinophils and basophiles.
iv. Mast cells
v. Dendritic cells
vi. Natural Killer cells
3. Non specific defenses of the host:
a) Species, race and individual resistance.
b) Age, sex, hormonal and nutritional influences.
4. Mechanism of non-specific defenses:
a) First line of defense – Physical, chemical and biological barriers
b) Second line of defense:
i. Humoral components: Defensins, pattern recognition proteins (PRP) and pathogen associated molecular patterns (PAMPs), complement, kinins, acute phase reactants.
ii. Cellular components: Phagocytic cells – PMNL, macrophages (reticulo-endothelial cell system) and dendritic cells
5. Functions: Phagocytosis (oxygen dependent and independent systems), Complement activation (General concept), Coagulation system, Inflammation (cardinal signs, mediators, vascular and cellular changes, role of Toll-like receptors)

Unit-II 1. Antigen

a) Concepts and factors affecting immunogenecity
b) Antigenic determinants, haptens and cross-reactivity, Carriers, Adjuvants
c) Types of antigens: Thymus-dependent and thymusindependent antigens, Synthetic antigens, Soluble and
particulate antigens, Autoantigens, Isoantigens, Species specific antigens, Organ specific antigens, Heterophile
antigens

2. Immunoglobulins

a) Structure of basic unit, chemical and biological properties
b) Characteristic of domain structure, functions of light and heavy chain domains
c) Molecular basis of antibody diversity (kappa chain, lambda chain and heavy chain diversity)
d) Classes of Immunoglobulins and their functions.

3. Organs of immune system

a) Primary lymphoid organs (Thymus and Bursa): Thymus – structure, thymic education (positive and negative
selection), Bone marrow
b) Secondary lymphoid organs – Structure and function of spleen and lymph node, mucous associated lymphoid
tissue and lymphatic system and lymph circulation

Unit- III 1. Adaptive / Acquired Immunity (Third line of defense):

a) Acquired immunity: Active and Passive immunity.
b) Primary and secondary response and its significance in vaccination programs
c) Clonal selection and clonal deletion (immune tolerance)
d) B cell biology, role of cytokines in activation and differentiation of B-cells

2. Cell Mediated Immune Response

a) Activation and differentiation of T cells
b) Mechanism of CTL mediated cytotoxicity, ADCC
c) Applications of CMI

3. T-cell biology

a) T-cell dependent antibody response. outline
b) T-cell independent antibody response. outline
c) Types of T-cells and Cluster of differentiation(CD)
d) T-cell receptor(TCR)

4. Cytokines

a) Definition and general characteristics
b) Types- colony stimulating factor, Interleukins, Tumor necrosis factor alpha

PAPER II MICROBIAL BIOTECHNOLOGY & RECOMBINANT DNA TECHNOLOGY

Unit-I Tools & Techniques of Genetic engineering

1. Introduction- Definition & scope of Biotechnology & Recombinant DNA technology.
2. Preparation of pure sample of DNA, enzymes used in DNA manipulation, analysis of DNA fragment size, joining of DNA fragments, vectors & their types
3. Introduction of rDNA into host cell, transformation of cells, identification of transformed cells, selection of clones – direct & indirect methods.
4. Expression of cloned genes, construction of gene library, cells for cloning, expression of prokaryotic genes.
5. PCR & its application, DNA finger printing.

Unit-II Health care Biotechnology

1. Production of hormones : Insulin
2. Production of Interferon
3. Production of vaccines : Conventional vaccines – BCG, Salk, Diptheria Toxoid , ATS, DNA Vaccine,Edible vaccines
4. Hybridoma technology, monoclonal antibody production
5. Gene Therapy

Unit- III Agricultural Biotechnology.

1. Protoplast fusion- Protist – Plant interaction, application of Agrobacterium tumifaeciens in gene transfer.
2. Production of Biopesticides – Buculo virus
3. Production of Biofertilizers – Rhizobium
4. Oriental Fermented food: Definition and production of soyasauce
5. Genetically modified foods- Definition and concept of goldenrice
6. Transgenic plants— Definition and concept of BT Cotton

Unit-IV Industrial Biotechnology

1. Biosensors—General concept of construction,Applications, Glucose sensor as an example.Concept of
Nanobiotechnology
2. Biochips- definition, example and applications
3. Enzyme technology- Applications of enzymes in industry, Production of industrial enzyme—amylase by deep tank & SSF, purification & recovery. Immobilized enzymes – applications & general methods of production- example
invertase immobilization.
4. Ethics & hazards of biotechnology

RTMNU Revised BSc Microbiology Sem 6 Syllabus 2021

PRACTICAL

  1. General guidelines for safety in microbiology laboratory, possible
    laboratory hazards, safety precautions and disposal of laboratory
    waste and ethics in microbiology.
  2. Study of permanent slides- T.S. of spleen, thymus, bursa of fabricius and lymphnode
  3.  Isolation of plasmid DNA (Major)
  4. Demonstration of restriction digestion
  5. Perform Quantitative WIDAL test
  6. Rapid plasma reagin (RPR) Test titre
  7. Blood Group Detection (Direct and Reverse typing)
  8. ASO/RA test
  9. Pregnancy test
  10. Perform Immuno diffusion (Major)
  11. ELISA Test
  12. Coomb’s Direct test
  13. Estimation of amylase (Major)
  14. Determination of Total viable Rhizobium and Azatobacter count in commercially available biofertilizers (Major)
  15. Immobilization of yeast and demonstration of invertase activity (Major)

Books Recommended For RTMNU Revised BSc Microbiology Sem 6 Syllabus 2021

1. Jawetz, Melnick and Adelberg’s Medical Microbiology, 26th Edition, Lange publication
2. Bacterial Pathogenesis –A molecular approach Abigail Salyer And Dixie Whitt 2nd Ed ASM press
3. Ananthanarayan and Panicker’s, Textbook of Microbiology, 9 edition
4. Kuby Immunology, 6 th Edition, W H Freeman and Company
5. Pathak & Palan, Immunology: Essential & Fundamental, 1st& 3rd Edition, Capital Publishing Company
6. Fahim Khan, Elements of Immunology, Pearson Education
7. Biotechnology by B.D. Singh
8. Baron Samuel , Medical Microbiology, 4 edition
9. Crueger W. and Crueger A. 2000 “Biotechnology -“A Textbook of Industrial Microbiology”, 2nd Edition, Panima Publishing Corporation, New Delhi.
10. R. C. Dubey, 2005 A Textbook of ‘’Biotechnology’’ S. Chand and Company, New Delhi